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C₁₈ column applicable in 100% aqueous mobile phase

We provide you with a solution for stable analysis for high polarity compound retention with 100% aqueous mobile phase.

Stable reproducibility with 100% aqueous mobile phase

Although retention can be increased by lowering the rate of organic solvent in the mobile phase with the C₁₈ column, reproducibility cannot be realized with 100% aqueous mobile phase.

CAPCELL PAK C₁₈ AQ addresses this by assuring good reproducibility even with 100% aquatic moble phase by optimizing the introduction of octadecyl group and polymer coating of the base packing material with a novel polymer.

Have you ever considered high polarity compound analysis using 100% aqueous mobile phase?

The CAPCELL PAK C₁₈ AQ column achieves good reproducibility with 100% aqueous mobile phase, and is durable as well.

Features

The highest surface polarity among C₁₈ columns

With the highest surface polarity among C₁₈columns, the AQ type enables strong retention and high separation of high polarity compounds. Furthermore, it enables the use of 100% aqueous mobile phase, which used to present problems in reproducibility with the conventional ODS columns.

Additionally, since it is coated with a polymer, the peak profile of basic compounds is free from tailings and, consequently, extremely good.

Hydrophobicity and surface polarity parameters of Osaka Soda columns

Excellent retention capacity for high polarity compounds

Designed for high surface polarity, CAPCELL PAK C₁₈ AQ is the only column among CAPSEL PAK products that enables stable analysis with 100% aqueous mobile phase. Moreover, it has excellent retention capacity for high polarity compounds.

Fig. 1Analysis of nucleic-acid bases

Fig. 1 Analysis of nucleic-acid bases

Column:: 4.6mm i.d.×150mm
Mobile phase:: 20mmol/L KH₂PO₄,
20mmol/L K₂HPO₄
Flow rate:: 1.0mL/min
Temperature:: 40°C
Detection:: UV254nm
Sample:: (1) Sodium sulfite (2) Cytosine
(3) Thymine (4) Uracil
(5) Adenine (6)Guanine

Fig. 2Analysis of water soluble vitamin B group

Fig. 2 Analysis of water soluble vitamin B group

Column:: CAPCELL PAK C₁₈ AQ S3
4.6mm i.d.×75mm
Mobile phase:: 10mmol/L CH₃COONH₄
Temperature:: 40°C
Flow rate:: 1.0mL/min
Detection:: UV254nm
Sample:: (1) Nicotinic acid
(2) Pyridoxal
(3) Pyridoxine
(4) Nicotinamide
(Concentration is 100μL/mL for all)
Injection volume:: 10μL

Higher-than-expected separation capacity even with 100% organic solvent mobile phase

Since the type of polymer coating and the introduction rate of C₁₈ have been modified to enable the use of 100% aqueous mobile phase, hydrophobic compounds can be eluted faster with 100% organic mobile phase.

Fig. 3Analysis of lipid soluble vitamins

Fig. 3 Analysis of lipid soluble vitamins

High resistance to acid and alkali

Poor durability is a problem in columns designed for use with 100% aqueous mobile phase. However, the AQ has been designed to improve resistance to acid and alkali with a polymer coating and the introduction of a unique C₁₈ group.

AQ shows excellent resistance to acid and alkali in comparison with other columns capable of using 100% aqueous mobile phase, in which the resistance tends to deteriorate quickly.

Fig. 4Results of durability test

Fig. 4 Results of durability test

Test conditions for acid resistance

Mobile phase:: (A)2vol% TFA,H₂O(pH1)
(B)2vol% TFA,CH₃CN
B 65% (20min) → 0% (60min)
→ 98% (5min) → 65% (5min)
Flow rate:: 1.0mL/min
Temperature:: 60°C
Detection:: UV254nm
Sample:: Uracil, amyl benzene

Test conditions for alkali resistance

Column:: 4.6mm i.d.×150mm
Mobile phase:: 4mmol/L Na₂B₄O₇/CH₃OH
=90/10 (pH10.0)
Flow rate:: 1.0mL/min
Temperature:: 50°C
Detection:: UV254nm
* Survival rate (%) of retention factor (k) of benzyl alcohol after 20 hours of dipping

Good peak profile also with basic compounds

The AQ type column comprises packing material coated with polymer; least influenced by silanol, and, consequently, is able to elute the basic compounds as a sharp peak without tailing.

Fig. 5Analysis of pyridine/phenol

Fig. 5 Analysis of pyridine/phenol

Column:: 4.6mm i.d.×150mm
Mobile phase:: CH₃CN/H₂O=30/70
Flow rate:: 1.0mL/min
Temperature:: 40°C
Detection:: UV254nm
Sample:: (1) Pyridine (2) Phenol
As:: Tailing factor

Realization of low pressure allows a length of 250 mm and a particle diameter of 3 µm

Through the selection of silica gel accurately graded for AQ, high number theory plate (NTP) in 5 and 3 µm particle with an amazingly low pressure became available.

The changeover from a particle of 5 µm to 3 µm has enabled improved separation and fast analysis. Which option would you prefer to pursue in the analysis of high polarity compounds?