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HPLC Column

CAPCELL PAK C18 AQ

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C18 column applicable in 100% aqueous mobile phase

We provide you with a solution for stable analysis for high polarity compound retention with 100% aqueous mobile phase.

Stable reproducibility with 100% aqueous mobile phase

Although retention can be increased by lowering the rate of organic solvent in the mobile phase with the C18 column, reproducibility cannot be realized with 100% aqueous mobile phase.

CAPCELL PAK C18 AQ addresses this by assuring good reproducibility even with 100% aquatic moble phase by optimizing the introduction of octadecyl group and polymer coating of the base packing material with a novel polymer.

Have you ever considered high polarity compound analysis using 100% aqueous mobile phase?

The CAPCELL PAK C18 AQ column achieves good reproducibility with 100% aqueous mobile phase, and is durable as well.

Features

The highest surface polarity among C18 columns

With the highest surface polarity among C18columns, the AQ type enables strong retention and high separation of high polarity compounds. Furthermore, it enables the use of 100% aqueous mobile phase, which used to present problems in reproducibility with the conventional ODS columns.

Additionally, since it is coated with a polymer, the peak profile of basic compounds is free from tailings and, consequently, extremely good.

Hydrophobicity and surface polarity parameters of Osaka Soda columns

Excellent retention capacity for high polarity compounds

Designed for high surface polarity, CAPCELL PAK C18 AQ is the only column among CAPSEL PAK products that enables stable analysis with 100% aqueous mobile phase. Moreover, it has excellent retention capacity for high polarity compounds.

Fig. 1Analysis of nucleic-acid bases

Fig. 1 Analysis of nucleic-acid bases

Column:
4.6mm i.d.×150mm
Mobile phase:
20mmol/L KH2PO4,
20mmol/L K2HPO4
Flow rate:
1.0mL/min
Temperature:
40°C
Detection:
UV254nm
Sample:
(1) Sodium sulfite (2) Cytosine
(3) Thymine (4) Uracil
(5) Adenine (6)Guanine

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Fig. 2Analysis of water soluble vitamin B group

Fig. 2 Analysis of water soluble vitamin B group

Column:
CAPCELL PAK C18 AQ S3
4.6mm i.d.×75mm
Mobile phase:
10mmol/L CH3COONH4
Temperature:
40°C
Flow rate:
1.0mL/min
Detection:
UV254nm
Sample:
(1) Nicotinic acid
(2) Pyridoxal
(3) Pyridoxine
(4) Nicotinamide
(Concentration is 100μL/mL for all)
Injection volume:
10μL

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Higher-than-expected separation capacity even with 100% organic solvent mobile phase

Since the type of polymer coating and the introduction rate of C18 have been modified to enable the use of 100% aqueous mobile phase, hydrophobic compounds can be eluted faster with 100% organic mobile phase.

Fig. 3Analysis of lipid soluble vitamins

Fig. 3 Analysis of lipid soluble vitamins

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High resistance to acid and alkali

Poor durability is a problem in columns designed for use with 100% aqueous mobile phase. However, the AQ has been designed to improve resistance to acid and alkali with a polymer coating and the introduction of a unique C18 group.

AQ shows excellent resistance to acid and alkali in comparison with other columns capable of using 100% aqueous mobile phase, in which the resistance tends to deteriorate quickly.

Fig. 4Results of durability test

Fig. 4 Results of durability test

Test conditions for acid resistance
Mobile phase:
(A)2vol% TFA,H2O(pH1)
(B)2vol% TFA,CH3CN
B 65% (20min) → 0% (60min)
→ 98% (5min) → 65% (5min)
Flow rate:
1.0mL/min
Temperature:
60°C
Detection:
UV254nm
Sample:
Uracil, amyl benzene
Test conditions for alkali resistance
Column:
4.6mm i.d.×150mm
Mobile phase:
4mmol/L Na2B4O7/CH3OH
=90/10 (pH10.0)
Flow rate:
1.0mL/min
Temperature:
50°C
Detection:
UV254nm
* Survival rate (%) of retention factor (k) of benzyl alcohol after 20 hours of dipping

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Good peak profile also with basic compounds

The AQ type column comprises packing material coated with polymer; least influenced by silanol, and, consequently, is able to elute the basic compounds as a sharp peak without tailing.

Fig. 5Analysis of pyridine/phenol

Fig. 5 Analysis of pyridine/phenol

Column:
4.6mm i.d.×150mm
Mobile phase:
CH3CN/H2O=30/70
Flow rate:
1.0mL/min
Temperature:
40°C
Detection:
UV254nm
Sample:
(1) Pyridine (2) Phenol
As:
Tailing factor

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Realization of low pressure allows a length of 250 mm and a particle diameter of 3 µm

Through the selection of silica gel accurately graded for AQ, high number theory plate (NTP) in 5 and 3 µm particle with an amazingly low pressure became available.

The changeover from a particle of 5 µm to 3 µm has enabled improved separation and fast analysis. Which option would you prefer to pursue in the analysis of high polarity compounds?

Fig. 6Column pressure

Fig. 6 Column pressure

Conditions for comparison of column pressure
Column size:
4.6mm i.d.×150mm
Mobile phase:
H2O/CH3OH=50/50
Flow rate:
1.0mL/min
Temperature:
40°C

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Fig. 7Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

Fig. 7 Analysis of nucleic bases/nucleoside

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Physical properties

Functional
group
Particle
diameter
(μm)
Pore
diameter
(nm)
Specific
surface area
(m2/g)
C% Density
(µmol/m2)
Range of
applicable pH
USP
Octadecyl group 3 8 330 12 1.7 2~9 L1
Octadecyl group 5 8 300 11 1.7 2~9 L1
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