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What is HILIC? |
| Hydrophilic interaction liquid chromatography (HILIC) is a relatively new LC technique that uses a hydrophilic stationary phase, in most cases, with organic-dominant mobile phase. The elution order of substances in HILIC mode is roughly the reverse of that in reversed-phase mode.
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What is PC? |
| Phosphorylcholine (PC) is a partial structure of phosphatidylcholine (lecitin), one of the phospholipids forming cell membranes. PC has a betaine structure and shows high hydrophilicity, biocompatibility, and inhibitory effect of protein adhesion. Its superhydrophilic character is suitable to the application as a HILIC phase. | 
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Features |  |
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| ·A silica-based HILIC column with phosphorylcholine (PC) group | ||||||
·Excellent retention and separation of very polar and hydrophilic compounds |
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·Large number of theoretical plates and outstanding peak profiles |
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·Also available in worldwide |
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 Strong retention of polar compounds |
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| HILIC mode provides another alternative to handle extremely polar and hydrophilic compounds, which are unretainable in reversed-phase (e.g. a chromatogram of allantoin, shown below) | ||||||
Amitriptyline, a compound with a strong basicity, is often used for discussing the quality of columns. PC HILIC provides excellent peak shapes for basic compounds, too. |
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| High sensitivity in LC/MS
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| In LC-MS of very hydrophilic or ionic compounds, HILIC columns are often chosen to avoid extremely water-rich, or ion-pair containing mobile phases used under reversed-phase mode. The preferable nature common to PC HILIC seems to be the use of mobile phases of higher-organic contents, which are advantageous in providing larger diffusion constants of analytes during their migration through the columns, and also better ionization efficiency in electrospray ionization (ESI)
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| PC HILIC shows large numbers of theoretical plates, compared to conventional HILIC columns.
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