Which DAISOGEL grade is best for small molecule purification?
The key for a good small molecule separation–or any chromatographic separation, for that matter–is that your target molecule has access to the entire surface area of the silica gel-based stationary phase. The pore size of the bead must be wide enough for this purpose, yet small enough for the silica product to have a large enough surface area to provide an effective loadability and ensure optimized process economics. In fact, the surface area and the pore size have direct correlation in DAISOGEL products.
For small molecules, there is one grade in particular that strikes the right balance between pore size and surface area. Our best choice for small molecules is the SP-100-10-ODS-P grade.
Porosity Comparison
DAISOGEL Grade
Pore Size (Å)
Pore Volume (mL/g)
Surface Area (m2/g)
SP-50
50
0.8
600
SP-100
100
1.1
450
SP-120
120
1.0
300
ODS Loadability Comparison
Silica Gel Grade
Maximum Loadability (mg)
SP-100-ODS-P
19.7
Kromasil-100-C18
10.6
Inertsil-ODS-3V
8.2
Symmetry-C18
5.8
KROMASIL® is a registered trademark of Nouryon in a number of territories in the world.
Inertsil™ is a trademark of GL Sciences, Inc.
Symmetry® is a registered trademark of Waters Corporation.
While loadability is critical for process economics of small molecule purification, resolution is also important in order to ensure separation and purity. Below we map the resolving power of various ODS phases versus their loadability. Ideally, the chromatographer wants a silica gel that offers high loading without sacrificing resolution. You can see that our design of the SP-100-10-ODS-P grade has resulted in a silica gel that finds the “sweet spot” of highest loadability as well as highest resolution.
Resolution to Loadability Correlation
An additional benefit of the ODS-P Series is the ultra-high pure nature of the bare silica gel due to its advanced residual silanol management. Its very low bleed makes this phase a safe choice, and the exceptional peak shape coming from a column packed with SP-100-10-ODS-P makes the separation of close-eluting impurities even easier!
Clearly the best candidate for efficient purification of small molecule APIs, we can full-heartedly recommend it!
Contact us to learn more
Contact us today to request a sample or check current pricing and availability of our SP-100-10-ODS-P silica gel for your small molecule purification.