HPLC Conditions
Mobile Phase:
(A) 2 vol% TFA in H
2
O (pH = 1)
(B) 2 vol% TFA in acetonitrile
B solution: 65% (20 min) → 0% (60 min) → 90% (5 min) → 65% (5 min)
Temperature:
60°C
Flow Rate:
1.0mL/min
Detection:
UV 254nm
Samples:
Uracil and amylbenzene
【Fig. 1 】 Acid resistance test
